OPA1 Polyclonal antibody

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  WB analysis using 32465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32465-1-AP (OPA1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 32465-1-AP

  IP result of anti-OPA1 (IP:32465-1-AP, 4ug; Detection:32465-1-AP 1:40000) with mouse brain tissue lysate 1840 ug.

OPA1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 32465-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

OPA1 gene, OPA 1, EC:3.6.5.5, Dynamin-like GTPase OPA1, short form, Dynamin-like GTPase OPA1, mitochondrial

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32465-1-AP targets OPA1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OPA1 fusion protein Ag38295
• Full Name: optic atrophy 1 (autosomal dominant)
• Calculated Molecular Weight: 960 aa, 112 kDa
• Observed Molecular Weight: 80-100 kDa
• GenBank Accession Number: BC075805
• Gene Symbol: OPA1
• Gene ID (NCBI): 4976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O60313
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms.