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  • KD/KO Validated

OPA1 Polyclonal antibody

OPA1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, rat, mouse and More (7)





Cat no : 27733-1-AP


KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, mouse brain tissue, rat brain tissue, HEK-293T cells, HeLa cells, HepG2 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27733-1-AP targets OPA1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Cited Reactivityhuman, mouse, rat, goat, Chicken, zebrafish, hamster, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPA1 fusion protein Ag26887
Full Name optic atrophy 1 (autosomal dominant)
Calculated molecular weight 960 aa, 112 kDa
Observed molecular weight 100 kDa, 80-90 kDa
GenBank accession numberBC075805
Gene symbol OPA1
Gene ID (NCBI) 4976
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.


Product Specific Protocols
WB protocol for OPA1 antibody 27733-1-APDownload protocol
IHC protocol for OPA1 antibody 27733-1-APDownload protocol
IP protocol for OPA1 antibody 27733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Mi (Verified Customer) (02-21-2023)

Works well in human adipocyte cells, we got clean bands at the expected size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: adipocytes