OPA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OPA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (8)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

137

Reviews (2)

Cat no : 27733-1-AP

Synonyms

KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1

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Validation Data Gallery

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  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 27733-1-AP

  WB result of OPA1 antibody (27733-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPA1 transfected HEK-293 cells.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27733-1-AP (OPA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 27733-1-AP

  IP result of anti-OPA1 (IP:27733-1-AP, 4ug; Detection:27733-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of mouse heart using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27733-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27733-1-AP (OPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue, HEK-293 cells, rat brain tissue, HEK-293T cells, HeLa cells, mouse liver tissue, HepG2 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse heart tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 131 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Product Information

27733-1-AP targets OPA1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human, mouse, rat, pig, chicken, zebrafish, bovine, hamster, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OPA1 fusion protein Ag26887
• Full Name: optic atrophy 1 (autosomal dominant)
• Calculated Molecular Weight: 960 aa, 112 kDa
• Observed Molecular Weight: 100 kDa, 80-90 kDa
• GenBank Accession Number: BC075805
• Gene Symbol: OPA1
• Gene ID (NCBI): 4976
• RRID: AB_2810292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPA1 is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. OPA1 localizes to the inner mitochondrial membrane and helps regulate mitochondrial stability and energy output. This protein also sequesters cytochrome c. OPA1 is associated with the inner membrane and protects cells from apoptosis by regulating inner membrane dynamics. Mutation of OPA1 causes the disease dominant optic atrophy, a degeneration of the retinal ganglion cells. OPA1 undergoes complex posttranscriptional regulation and posttranslational proteolysis. OPA1 is regulated by proteolytic cleavage, which degrades long OPA1 isoforms into short isoforms. The gene OPA1 can be cleaved into some chains with MW 100 kDa and 80-90 kDa.

Publications

Species	Application	Title
human	CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
mouse	WB	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
mouse	WB	Acta Pharm Sin B; Honokiol alleviated neurodegeneration by reducing oxidative stress and improving mitochondrial function in mutant SOD1 cellular and mouse models of amyotrophic lateral sclerosis; Authors - Yujun Zhou
chicken	WB	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

P (Verified Customer) (09-23-2024)

excellent!

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEK

OPA1 Antibody Western Blot validation (1000 dilution) in HEK (Cat no:27733-1-AP)

FH

Mi (Verified Customer) (02-21-2023)

Works well in human adipocyte cells, we got clean bands at the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes