ONECUT1 Polyclonal antibody

ONECUT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25137-1-AP

Synonyms

Hepatocyte nuclear factor 6, HNF 6, HNF6, HNF6A, One cut domain family member 1, one cut homeobox 1, ONECUT1



Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25137-1-AP is ONECUT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ONECUT1 fusion protein Ag18250
Full Name one cut homeobox 1
Calculated Molecular Weight 465 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC140830
Gene Symbol ONECUT1
Gene ID (NCBI) 3175
RRIDAB_2879918
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.

Protocols

Product Specific Protocols
WB protocol for ONECUT1 antibody 25137-1-APDownload protocol
IHC protocol for ONECUT1 antibody 25137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

REVERBa couples the circadian clock to hepatic glucocorticoid action.

Authors - Giorgio Caratti
humanWB

J Virol

Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6.

Authors - Ruidong Hao
humanWB

Virol Sin

Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters

Authors - Xinna Ma
mouseIF

bioRxiv

Enhanced Plasmid-Based Transcriptional Activation in Developing Mouse Photoreceptors

Authors - Brendon M Patierno