ONECUT1 Polyclonal antibody

ONECUT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25137-1-AP

Synonyms

Hepatocyte nuclear factor 6, HNF 6, HNF6, HNF6A, One cut domain family member 1, one cut homeobox 1, ONECUT1

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Validation Data Gallery

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  WB analysis of mouse liver using 25137-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25137-1-AP (ONECUT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 25137-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25137-1-AP is ONECUT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ONECUT1 fusion protein Ag18250
• Full Name: one cut homeobox 1
• Calculated Molecular Weight: 465 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC140830
• Gene Symbol: ONECUT1
• Gene ID (NCBI): 3175
• RRID: AB_2879918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; REVERBa couples the circadian clock to hepatic glucocorticoid action.; Authors - Giorgio Caratti
human	WB	J Virol; Inhibition of Hepatitis B Virus Gene Expression and Replication by Hepatocyte Nuclear Factor 6.; Authors - Ruidong Hao
human	WB	Virol Sin; Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters; Authors - Xinna Ma
mouse	IF	bioRxiv; Enhanced Plasmid-Based Transcriptional Activation in Developing Mouse Photoreceptors; Authors - Brendon M Patierno