OLIG2 (for WB only) Monoclonal antibody

OLIG2 (for WB only) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

1E8C5

Cat no : 66513-1-Ig

Synonyms

BHLHB1, bHLHe19, OLIG2, OLIGO2, PRKCBP2, RACK17

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Validation Data Gallery

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  WB analysis of human brain using 66513-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 66513-1-Ig

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66513-1-Ig (OLIG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human brain tissue, human cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

66513-1-Ig targets OLIG2 (for WB only) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: OLIG2 (for WB only) fusion protein Ag18838
• Full Name: oligodendrocyte lineage transcription factor 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 35-39 kDa
• GenBank Accession Number: BC047511
• Gene Symbol: OLIG2
• Gene ID (NCBI): 10215
• RRID: AB_2881876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OLIG2, also named as BHLHB1, BHLHE19, PRKCBP2 and RACK17, is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1, OLIG2 establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. OLIG2 is widely expressed in subsets of glia cells and progenitors, and it is strongly induced at different sites by both acute and chronic injury, albeit with different mechanisms. OLIG2 acts as a repressor of neurogenesis in cells reacting to brain injury. It may represent an effective approach towards evoking neuronal repair from parenchymal precursors.(PMID:19390819 )

Publications

Species	Application	Title
		Cell Metab; Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK; Authors - Yajuan Li
human	WB	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
human	WB	Cell Biochem Funct; Ring finger protein 216 loss-of-function induces white matter hyperintensities by inhibiting oligodendroglia proliferation; Authors - Wenqing Xu
human	WB	Cell Metab; Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation; Authors - Jian Zhou
	WB	Commun Biol; Selenoprotein W modulates tau homeostasis in an Alzheimer's disease mouse model; Authors - Bingyu Ren
human	WB	Nucleic Acids Res; A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma; Authors - Xianli Du