OLIG2 Polyclonal antibody, PBS Only

OLIG2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25754-1-PBS

Synonyms

OLIG 2, Class E basic helix-loop-helix protein 19, Class B basic helix-loop-helix protein 1, bHLHe19, bHLHb1

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Validation Data Gallery

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  WB analysis of rat brain using 25754-1-AP (same clone as 25754-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25754-1-AP (OLIG2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 25754-1-AP (same clone as 25754-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 25754-1-AP (OLIG2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 25754-1-AP (same clone as 25754-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25754-1-AP (OLIG2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (25754-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 25754-1-AP (same clone as 25754-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using OLIG2 antibody (25754-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25754-1-PBS in a different storage buffer formulation.

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Product Information

25754-1-PBS targets OLIG2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OLIG2 fusion protein Ag22642
• Full Name: oligodendrocyte lineage transcription factor 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC047511
• Gene Symbol: OLIG2
• Gene ID (NCBI): 10215
• RRID: AB_2880226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.