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OLFM4 Polyclonal antibody

OLFM4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28432-1-AP

Synonyms

Antiapoptotic protein GW112, bA209J19.1, GC1, GW112, hGC 1, hOLfD, KIAA4294, olfactomedin 4, OlfD, OLFM4, OLM4, UNQ362

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human small intestine tissue using OLFM4 Polyclonal antibody (28432-1-AP)

    IHC staining of human small intestine using 28432-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28432-1-AP (OLFM4 antibody) at dilution of 1:1000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using OLFM4 Polyclonal antibody (28432-1-AP)

    IHC staining of human small intestine using 28432-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 28432-1-AP (OLFM4 antibody) at dilution of 1:1000 (under 40x lens).

Tested Applications

Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

28432-1-AP targets OLFM4 in IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OLFM4 fusion protein Ag28914
Full Name olfactomedin 4
Calculated Molecular Weight 57 kDa
GenBank Accession NumberNM_006418
Gene Symbol OLFM4
Gene ID (NCBI) 10562
RRIDAB_2918163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Olfactomedin-4 (OLFM4), also named as hGC-1 (human G-CSF-stimulated clone-1) or antiapoptotic protein GW112, is a secreted glycoprotein normally expressed in bone marrow, prostate, small intestine, stomach, colon and pancreas (PMID: 11867215; PMID: 22471589). OLFM4 has been found to be up-regulated in gastrointestinal cancer and inflammatory disease including inflammatory bowel disease and H. pylori infection (PMID: 20534456). OLFM4 is involved in cellular process such as apoptosis, cell adhesion and tumor growth (PMID: 22471589; 16566923; 15059901).

Protocols

Product Specific Protocols
IHC protocol for OLFM4 antibody 28432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Rep

An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation

Authors - Ketty Sinigaglia
View Article