OLFM1 Polyclonal antibody

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  IHC staining of human lung cancer using 10079-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10079-1-AP (OLFM1 antibody) at dilution of 1:50 (under 10x lens).

OLFM1 Polyclonal Antibody for IHC, ELISA

Cat No. 10079-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

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Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10079-1-AP targets OLFM1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OLFM1 fusion protein Ag0125
• Full Name: olfactomedin 1
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: BC000189
• Gene Symbol: OLFM1
• Gene ID (NCBI): 10439
• RRID: AB_2157220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99784
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Olfactomedin 1 (Olfm1), also known as noelin and pancortin, is a secreted glycoprotein belonging to a family of olfactomedin domain-containing proteins. The gene of Olfm1 is located on chromosome 9q34.3. Four variant transcripts (AMY, BMY, AMZ, and BMZ) are produced by alternative splicing. Olfm1 may be involved in the regulation of neural crest cell production, neuronal differentiation and ischemic neuronal death in adult. This antibody is specific for isoform 3 and isoform 4 (AMY) of Olfm1 with native molecular weights of 54 kDa and 16 kDa respectively. AMY may have an essential role in nerve tissue.