OIP5 Monoclonal antibody

• Featured Product
• KD/KO Validated

OIP5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E9B5

Cat no : 66461-1-Ig

Synonyms

5730547N13Rik, Cancer/testis antigen 86, CT86, LINT 25, MIS18B, MIS18beta, OIP 5, OIP5, Opa interacting protein 5, Protein Mis18 beta

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Validation Data Gallery

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  WB analysis using 66461-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66461-1-Ig (OIP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66461-1-Ig

  WB result of OIP5 antibody (66461-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-OIP5 transfected Jurkat cells.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, COLO 320 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

66461-1-Ig targets OIP5 in WB, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: OIP5 fusion protein Ag2790
• Full Name: Opa interacting protein 5
• Calculated Molecular Weight: 229 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC015050
• Gene Symbol: OIP5
• Gene ID (NCBI): 11339
• RRID: AB_2881830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OIP5, also named as IS18B and CT86, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. It is over expressed in the majority of primary lung and esophageal cancers. OIP5 is a promising target for developing new prognostic biomarkers and anti-cancer drugs.

Publications

Species	Application	Title
human	WB,IP,CoIP	Oncogene; Engineered targeting OIP5 sensitizes bladder cancer to chemotherapy resistance via TRIP12-PPP1CB-YBX1 axis; Authors - Xianteng Wang ; KD Validated