OGT Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

OGT Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B2A6

Cat no : 66823-1-PBS

Synonyms

FLJ23071, HRNT1, O GLCNAC, OGT

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Validation Data Gallery

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  WB analysis using 66823-1-Ig (same clone as 66823-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66823-1-Ig (OGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66823-1-Ig (OGT antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66823-1-Ig (same clone as 66823-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using OGT antibody (66823-1-Ig, Clone: 2B2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66823-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66823-1-PBS targets OGT in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: OGT fusion protein Ag28402
• Full Name: O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
• Calculated Molecular Weight: 1046 aa, 117 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC014434
• Gene Symbol: OGT
• Gene ID (NCBI): 8473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

O-linked N-acetylglucosamine transferase (OGT) catalyzes the attachment of N-acetylglucosamine (GlcNAc) monosaccharides to the hydroxyl group of serine or threonine residues of numerous nuclear and cytoplasmic proteins and may play important roles in a large number of diverse intracellular processes ranging from translational control, transcription, transcriptional repression, INS resistance and regulation of the cell cycle. It exists as a heterotrimeric complex with two 110 kDa and one 70 kDa subunits. Recent studies have shown that O-GlcNAcylation plays essential roles in cancer formation and progression. O-GlcNAcylation as well as OGT expression was found to be significantly elevated in the cancer tissues.