OGFOD1 Polyclonal antibody

• 

  WB analysis using 30800-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30800-1-AP (OGFOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

OGFOD1 Polyclonal Antibody for WB, ELISA

Cat No. 30800-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TPA1, Prolyl 3-hydroxylase OGFOD1, OGFOD 1, KIAA1612, EC:1.14.11.-

---

Tested Applications

• Positive WB detected in: HeLa cells, DU 145 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30800-1-AP targets OGFOD1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OGFOD1 fusion protein Ag34034
• Full Name: 2-oxoglutarate and iron-dependent oxygenase domain containing 1
• Calculated Molecular Weight: 542 aa, 63 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC032919
• Gene Symbol: OGFOD1
• Gene ID (NCBI): 55239
• RRID: AB_3086419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N543
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 (OGFOD1) is a proly hydroxylase which belongs to 2-OG-Fe (II) dioxygenase family, it also namely KIAA1612 and TPA1. OGFOD1 is expressed in many tissues, mainly located in the nucleoplasm. It increased in many cancers and regulates both transcription and translation. The apparent molecular weight is larger than the calculated molecular weight of 63 kDa, which is likely due to posttranslational modification, presumably by glycosylation. (PMID:34298635 and 20579638)