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ODC1 Recombinant antibody

ODC1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230339H4

Cat no : 83108-1-RR

Synonyms

ODC, EC:4.1.1.17, 230339H4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ODC1 Recombinant antibody (83108-1-RR)

    IF Staining of HeLa using 83108-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ODC1 antibody (83108-1-RR, Clone: 230339H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of U2OS cells using ODC1 Recombinant antibody (83108-1-RR)

    FC experiment of U2OS using 83108-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human ODC1 (83108-1-RR, Clone:230339H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83108-1-RR

    Affinity and Kinetic Characterization of 83108-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83108-1-RR against Human ODC1 were performed. The affinity constant is 172 nM.

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83108-1-RR targets ODC1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ODC1 fusion protein Ag29751
Full Name ornithine decarboxylase 1
Calculated Molecular Weight 461 aa, 51 kDa
GenBank Accession NumberBC025296
Gene Symbol ODC1
Gene ID (NCBI) 4953
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ODC1 antibody 83108-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols