ODC1 Polyclonal antibody

ODC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23454-1-AP

Synonyms

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

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Validation Data Gallery

• 

  WB analysis of RAW264.7 using 23454-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23454-1-AP (ODC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 23454-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23454-1-AP (ODC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 23454-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 23454-1-AP (ODC1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: RAW264.7 cells
• Positive IHC detected in: human prostate hyperplasia tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23454-1-AP targets ODC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ODC1 fusion protein Ag10927
• Full Name: ornithine decarboxylase 1
• Calculated Molecular Weight: 461 aa, 51 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC025296
• Gene Symbol: ODC1
• Gene ID (NCBI): 4953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.