OCIAD2 Polyclonal antibody

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  WB analysis using 13437-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 13437-1-AP (OCIAD2 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 13437-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13437-1-AP (OCIAD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13437-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13437-1-AP (OCIAD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 13437-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13437-1-AP (OCIAD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 13437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using OCIAD2 antibody (13437-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

OCIAD2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13437-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ovarian carcinoma immunoreactive antigen-like protein, OCIA domain-containing protein 2, OCIA domain containing 2

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Tested Applications

• Positive WB detected in: U-251 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13437-1-AP targets OCIAD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OCIAD2 fusion protein Ag4243
• Full Name: OCIA domain containing 2
• Calculated Molecular Weight: 99 aa, 11 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC032808
• Gene Symbol: OCIAD2
• Gene ID (NCBI): 132299
• RRID: AB_3085414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q56VL3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.