Various lysates were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 14809-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14809-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14809-1-AP
IP result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.
IP result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.
IHC staining of human ovary tumor using 14809-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 14809-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary tumor using 14809-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 14809-1-AP
Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human ovary tumor tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Hela cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:12000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
OBFC2B, also named as NABP2 or SSB1, is a 211 amino acid protein, which belongs to the SOSS-B family. OBFC2B localizes to nuclear foci following DNA damage. OBFC2B is a component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, OBFC2B acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The calculated molecular weight of OBFC2B is 22 kDa, but modified OBFC2B is about 32 kDa.
Various lysates were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 14809-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14809-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14809-1-AP (OBFC2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary tumor using 14809-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 14809-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 14809-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary tumor using 14809-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14809-1-AP (OBFC2B Antibody) at dilution of 1:50 (under 10x lens).
IP Figures
IP experiment of HeLa using 14809-1-AP
IP result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.
IP experiment of HeLa using 14809-1-AP
IP result of anti-OBFC2B (IP:14809-1-AP, 4ug; Detection:14809-1-AP 1:500) with HeLa cells lysate 3200ug.
IF/ICC Figures
IF Staining of HeLa using 14809-1-AP
Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 14809-1-AP
Immunofluorescent analysis of Hela cells, using OBFC2B antibody 14809-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
OBFC2B Polyclonal antibody
Catalog Number
14809-1-AP
Citations
3
Dilutions
WB : 1:2000-1:12000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
