OBFC2A Polyclonal antibody

• Featured Product
• KD/KO Validated

OBFC2A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16719-1-AP

Synonyms

hSSB2, OBFC2A, Sensor of ssDNA subunit B2, SOSS B2, SOSS complex subunit B2, SSB2

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Validation Data Gallery

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  WB analysis of COLO 320 using 16719-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16719-1-AP (OBFC2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16719-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16719-1-AP (OBFC2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Product Information

16719-1-AP targets OBFC2A in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OBFC2A fusion protein Ag10158
• Full Name: oligonucleotide/oligosaccharide-binding fold containing 2A
• Calculated Molecular Weight: 124 aa, 14 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC107723
• Gene Symbol: OBFC2A
• Gene ID (NCBI): 64859
• RRID: AB_2156021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Single-stranded DNA (ssDNA)-binding proteins (SSBs) are ubiquitous and essential for a variety of DNA metabolic processes, including recombination, replication, and detection and repair of damage. SSBs have multiple roles in binding and sequestering ssDNA, detecting DNA damage, stimulating nucleases, helicases and strand-exchange proteins, activating transcription and mediating protein-protein interactions [PMID:18449195] Human single-stranded DNA-binding protein 1 (hSSB1), encoded by OBFC2B, is characterized as an essential factor for the initiation of DNA damage checkpoints and the maintenance of genomic stability [PMID:22940690].

Publications

Species	Application	Title
mouse	WB	Blood; Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress.; Authors - Wei Shi
human	WB	Cell Res; The Integrator complex controls the termination of transcription at diverse classes of gene targets.; Authors - Jeffrey R Skaar ; KD Validated
mouse	WB	EMBO J; The hSSB1 orthologue Obfc2b is essential for skeletogenesis but dispensable for the DNA damage response in vivo.; Authors - Feldhahn Niklas N ; KD Validated
human	WB	Front Oncol; The Potential Prognostic Role of Oligosaccharide-Binding Fold-Containing Protein 2A (OBFC2A) in Triple-Negative Breast Cancer.; Authors - Qianxue Wu
mouse	WB	J Immunol; SSB1/SSB2 Proteins Safeguard B Cell Development by Protecting the Genomes of B Cell Precursors.; Authors - Matthias Pfeifer ; KO Validated
human,mouse	IP	Sci Rep; hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage.; Authors - Didier Boucher