OATL1 Polyclonal antibody

OATL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28597-1-AP

Synonyms

TBC1D25, TBC1 domain family, member 25, TBC1 domain family member 25

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Validation Data Gallery

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  WB analysis using 28597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28597-1-AP (OATL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28597-1-AP (OATL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28597-1-AP targets OATL1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OATL1 fusion protein Ag29470
• Full Name: TBC1 domain family, member 25
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC101817
• Gene Symbol: TBC1D25
• Gene ID (NCBI): 4943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OATL1, also known as TBC1D25, is a protein with a TBC domain and functions as a Rab GTPase activating protein. It is involved in the fusion of autophagosomes with endosomes and lysosomes. OATL1 is recruited to isolation membranes and autophagosomes through direct interaction with Atg8 homologues and is involved in the fusion between autophagosomes and lysosomes through its GAP activity.