OAS2 Polyclonal antibody

OAS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14214-1-AP

Synonyms

(2 5)oligo(A) synthase 2, 2 5 oligoadenylate synthase 2, 2 5A synthase 2, OAS2, p69 OAS / p71 OAS, p69OAS / p71OAS

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Validation Data Gallery

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  WB analysis of Jurkat using 14214-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14214-1-AP (OAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14214-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14214-1-AP (OAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14214-1-AP (OAS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14214-1-AP (OAS2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14214-1-AP targets OAS2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OAS2 fusion protein Ag5436
• Full Name: 2'-5'-oligoadenylate synthetase 2, 69/71kDa
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 66-71 kDa
• GenBank Accession Number: BC049215
• Gene Symbol: OAS2
• Gene ID (NCBI): 4939
• RRID: AB_10892671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS2, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS2 is also named as p69 OAS/p71 OAS. It has 3 isoforms(71/69/20 kDa) produced by alternative splicing. The OAS2 isozymes are 69-71 kDa and form dimers(PMID:17765707). Glycosylation is essential for its activity(PMID:9880569). Constitutive expression of the 69-kDa form of the OAS2 protein in human cells inhibited the replication of encephalomyocarditis virus (EMCV) but not that of VSV, Sendai virus, or reovirus(PMID:16501108). This antibody recognizes isoform p71 and isoform p69 of OAS2.