OAS1 Recombinant antibody, PBS Only

OAS1 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H4

Cat no : 82883-1-PBS

Synonyms

(2 5)oligo(A) synthase 1, 2 5 oligoadenylate synthase 1, 2 5A synthase 1, E18/E16, IFI 4, OAS1, OIAS, OIASI, p46/p42 OAS

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Validation Data Gallery

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  WB analysis using 82883-1-RR (same clone as 82883-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82883-1-RR (OAS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 82883-1-RR (same clone as 82883-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 82883-1-RR (OAS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 82883-1-RR (same clone as 82883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 82883-1-RR (same clone as 82883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 82883-1-RR (same clone as 82883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 82883-1-RR (same clone as 82883-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82883-1-RR (OAS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82883-1-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 82883-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82883-1-RR against Human OAS1 were performed. The affinity constant is below 1 pM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82883-1-PBS targets OAS1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OAS1 fusion protein Ag6793
• Full Name: 2',5'-oligoadenylate synthetase 1, 40/46kDa
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000562
• Gene Symbol: OAS1
• Gene ID (NCBI): 4938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The 2-prime,5-prime oligoadenylate synthetases (OASs), such as OAS1, are interferon-induced proteins characterized by their capacity to catalyze the synthesis of 2-prime,5-prime oligomers of adenosine (2-5As). OAS1 (type I enzymes) has some isoforms with the MW of 40, 42, 44, 46, and 48 kDa (PMID:12590567, 19383565). OAS1 is a strong candidate for determining susceptibility or resistance to viral infections(PMID:15732009).