O-GlcNAc Monoclonal antibody

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  WB analysis using 65292-1-Ig

  Untreated or PUGNAc (an inhibitor of N-acetyl-β-D-glucosaminidase) treated HeLa cells were subjected to SDS PAGE followed by western blot with 65292-1-Ig (O-GlcNAc antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

O-GlcNAc Monoclonal Antibody for WB

Cat No. 65292-1-Ig

Clone No.CTD110.6

Host / Isotype

Mouse / IgM

Reactivity

n/a

Applications

WB

Synonyms

O-glycosylation, O-Glc, O Glc, glycosylation, O-linked β-N-acetylgulcosamine

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Tested Applications

• Positive WB detected in: HeLa cells

For this antibody, it is recommended to use BSA as the blocking agent, avoiding using milk in the blocking or dilution buffers.

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65292-1-Ig targets O-GlcNAc in WB applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: O-GlcNAc
• RRID: AB_3670285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

O-GlcNAc (O-linked β-N-acetylgulcosamine) is a post-translational modification of serine and threonine residues in proteins. O-GlcNAc modification is abundant in all multicellular eukaryotes, exclusively found on nuclear and cytoplasmic proteins rather than membrane proteins and secretory proteins (PMID: 11269319; 10924527). O-GlcNAc is attached to the protein backbone by enzymatic addition of the N-acetylgulcosamine (GlcNAc) moiety of uridine 5′-diphospho (UDP)-GlcNAc to the hydroxyl oxygen of serines or threonines by the O-linked β-N-acetylgulcosamine transferase (OGT). O-GlcNAc glycosylated proteins can be reversiblely deglycosylated by β-D-N-acetylgulcosaminase (O-GlcNAcase) (PMID: 21526146). O-GlcNAc modification couples many biological processes and plays important roles in development, normal physiology and physiopathology (PMID: 32349769).