Nurr1/NR4A2 Polyclonal antibody, PBS Only

Nurr1/NR4A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10975-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 10975-2-AP (same clone as 10975-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 10975-2-AP (same clone as 10975-2-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

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Product Information

10975-2-PBS targets Nurr1/NR4A2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nurr1/NR4A2 fusion protein Ag1418
• Full Name: nuclear receptor subfamily 4, group A, member 2
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC009288
• Gene Symbol: Nurr1
• Gene ID (NCBI): 4929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].