ChromoTek Nuclear Actin-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.

Specificity

Actin

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagGFP2

Publications

15

Cat no : acg-n

Synonyms

Actin Chromobody, Chromobody

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Validation Data Gallery

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  Nuclear Actin-Chromobody: NIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody (TagGFP2, green) and the Lamin-Chromobody (TagRFP, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum. Courtesy of Matthias Plessner.

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Product Information

DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.

• Description: The nuclear Actin-Chromobody allows imaging of actin dynamics in real-time in living cells.

  • Easily transfect your cells and non-invasively visualize actin cytoskeleton in real-time

  • Target and trace microfilaments in your cell line of interest

  • Use Actin-Chromobody as a marker of cellular morphology and as a probe for cellular motility

  • Actin-Chromobody has no notable effect cell viability or migration

  The nuclear Actin-Chromobody® plasmid has been published and described by Prof. Dr. Robert Grosse, Institute of Pharmacology, BPC, University of Marburg, Germany under license from ChromoTek:Matthias Plessner, Michael Melak, Pilar Chinchilla, Christian Baarlink, Robert Grosse (2015). Nuclear F-actin formation and reorganization upon cell spreading J Biol Chem. (Pubmed)
• Applications: Live cell imaging; Visualizing actin dynamics; Actin as biomarker or control
• Specificity: Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
• Reporter: TagGFP2
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Codon usage: Mammalian
• Selection: Kan/Neo
• Nuclear localization signal (NLS): Yes
• Sequence: With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Nature; Cytoplasmic control of intranuclear polarity by human cytomegalovirus.; Authors - Dean J Procter
	Nat Cell Biol; Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress.; Authors - Noa Lamm
	Nat Struct Mol Biol; The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling.; Authors - Kilian R Knoll
	Mol Cell; DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C; Authors - Marton Tibor Kovacs
	Nat Commun; Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing.; Authors - Baolei Yuan
IF	J Cell Sci; The prophase oocyte nucleus is a homeostatic G-actin buffer.; Authors - Kathleen Scheffler