ChromoTek Nuclear Actin-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

Actin

Publications

7

Cat no : acg-n

Synonyms

Actin Chromobody, Chromobody

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Validation Data Gallery

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  The time lapse analysis reveals the reorganization of actin after treatment with Cytochalasin D: HeLa cells were subjected to confocal imaging upon treatment with 2 µM of Cytochalasin D for 1 hour and recovery for 4 hours. Actin Chromobody enables monitoring of actin dynamics in real-time in living cells.

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Product Information

DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.

• Applications: Live cell imaging
• Specificity: Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Reporter: TagGFP2
• Codon usage: Mammalian
• Selection: Kan/Neo
• Encoded Nanobody/ VHH: Monoclonal anti-Actin single domain antibody (sdAb) fragment
• Sequence: With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
IF	Cell Death Dis; Targeting actin inhibits repair of doxorubicin-induced DNA damage: a novel therapeutic approach for combination therapy.; Authors - Lisa Pfitzer
	Nat Struct Mol Biol; The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling.; Authors - Kilian R Knoll
	Anat Rec (Hoboken); Nuclear Actin: From Discovery to Function.; Authors - Daniel J Kelpsch
	Genes Cells; Characterization of phalloidin-negative nuclear actin filaments in U2OS cells expressing cytoplasmic actin-EGFP.; Authors - Akira Nagasaki
	J Cell Sci; Nuclear F-actin and lamin a antagonistically modulate nuclear shape.; Authors - Sampada Mishra
	Nat Cell Biol; Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress.; Authors - Noa Lamm