ChromoTek Nuclear Actin-Chromobody® plasmid (TagGFP)
DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.
Vector type
Mammalian expression vector
Promoter
Constitutive CMV IE
Reporter
TagGFP2
Epitope
Actin
Cat no : acg-n
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.
Applications | Live cell imaging |
Specificity | Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii |
Vector type | Mammalian expression vector |
Promoter | Constitutive CMV IE |
Reporter | TagGFP2 |
Codon usage | Mammalian |
Selection | Kan/Neo |
Encoded Nanobody/ VHH | Monoclonal anti-Actin single domain antibody (sdAb) fragment |
Sequence | With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence. |
Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED |
Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Documentation
SDS |
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SDS Nuclear Actin-Chromobody® TagGFP plasmid (PDF) |
SDS Actin-Chromobody® TagGFP plasmid (PDF) |
Datasheet |
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Datasheet Actin-Chromobody® TagRFP plasmid (PDF) |
Datasheet Nuclear Actin-Chromobody® TagGFP plasmid (PDF) |
Datasheet Actin-Chromobody® TagGFP plasmid (PDF) |
Brochure |
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Chromotek nanobodies brochure (PDF) |
Publications
Application | Title |
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Cell Death Dis Targeting actin inhibits repair of doxorubicin-induced DNA damage: a novel therapeutic approach for combination therapy. | |
Nat Struct Mol Biol The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling. | |
Genes Cells Characterization of phalloidin-negative nuclear actin filaments in U2OS cells expressing cytoplasmic actin-EGFP. | |
Nat Cell Biol Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress. |