Notch1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Notch1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

46

Cat no : 10062-2-AP

Synonyms

p300, Notch 1, NOTCH, NICD, Motch

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Validation Data Gallery

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  WB analysis using 10062-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10062-2-AP (Notch1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10062-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10062-2-AP (Notch1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10062-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10062-2-AP (Notch1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10062-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10062-2-AP (Notch1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 32 publications below
• IHC: See 8 publications below
• IF: See 11 publications below
• IP: See 3 publications below

Product Information

10062-2-AP targets Notch1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Notch1 fusion protein Ag0107
• Full Name: Notch gene homolog 1 (Drosophila)
• Calculated Molecular Weight: 272 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC138441
• Gene Symbol: Notch1
• Gene ID (NCBI): 18128
• RRID: AB_2153338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOTCH1, also named as TAN1, belongs to the NOTCH family. NOTCH1 functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH1 affects the implementation of differentiation, proliferation and apoptotic programs. It may be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. NOTCH1 is involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV).

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. The antibody is specific to NOTCH1. It can recognize the full length NOTCH1(270 kDa) and cleaved NOTCH1 forms 120 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
mouse	WB, IP	Nat Commun; TRPC6 specifically interacts with APP to inhibit its cleavage by γ-secretase and reduce Aβ production.; Authors - Junfeng Wang
human	WB	Autophagy; Engagement of circular RNA HECW2 in the nonautophagic role of ATG5 implicated in the endothelial-mesenchymal transition.; Authors - Li Yang
human	IF	J Nanobiotechnology; Vasorin-containing small extracellular vesicles retard intervertebral disc degeneration utilizing an injectable thermoresponsive delivery system; Authors - Zhiwei Liao
human	WB,IHC	Oncotarget; Alterations of tumor microenvironment by carbon monoxide impedes lung cancer growth.; Authors - Zsuzsanna Nemeth
human	IHC	Cell Death Dis; POFUT1 promotes colorectal cancer development through the activation of Notch1 signaling.; Authors - Yuheng Du