Nlrp3/NLRP3 Polyclonal antibody

Nlrp3/NLRP3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 30109-1-AP

Synonyms

Nlrp3, NALP3, MWS, Mmig1, AII/AVP

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Validation Data Gallery

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  WB analysis using 30109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 30109-1-AP

  Non-treated RAW 264.7 cells and LPS-treated RAW 264.7 cells lysates were subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30109-1-AP

  rat spleen tissue was subjected to SDS PAGE followed by western blot with 30109-1-AP (Nlrp3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 30109-1-AP

  IP result of anti-Nlrp3 (IP:30109-1-AP, 4ug; Detection:30109-1-AP 1:4000) with RAW 264.7 cells lysate 1200 ug.

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  IF Staining of HepG2 using 30109-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Nlrp3 antibody (30109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: J774A.1 cells, rat spleen tissue, RAW 264.7 cells, LPS treated RAW 264.7 cells
• Positive IP detected in: RAW 264.7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

30109-1-AP targets Nlrp3/NLRP3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nlrp3/NLRP3 fusion protein Ag32515
• Full Name: NLR family, pyrin domain containing 3
• Calculated Molecular Weight: 118kd
• Observed Molecular Weight: 108-120 kDa
• GenBank Accession Number: NM_145827
• Gene Symbol: Nlrp3
• Gene ID (NCBI): 216799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NLRP3, also named as NALP3, CIASI, C1orf7, PYPAF1, Cryopyrin and MIM606416, belongs to NLR family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NLRP3 inflammasome is expressed in immune cells, including monocytes, macrophages, and dendritic cells. When the NLRP3 inflammasome is activated, the PYD domain of NLRP3 mediates recruitment of an adaptor protein called ASC and the effector protein procaspase-1 to form an NLRP3 inflammasome complex that can cleave inactive procaspase-1 to from active caspase-1. And then the active caspase-1 results in secretion of interleukin (IL)-18 and IL-1β. Activation of the NLRP3 inflammasome is also required for HMGB1 secretion. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (PMID:27669650). NLRP3 has some isoforms with the MW of 106-118 kDa and 75-83 kDa. An additional 130-135 kDa band could also be detected (PMID: 17164409; PMID: 34680443; PMID: 35585627). This antibody is specific for mouse Nlrp3.

Publications

Species	Application	Title
human	WB	JACC Basic Transl Sci; PCSK9 Promotes Hypoxia-Induced EC Pyroptosis by Regulating Smac Mitochondrion-Cytoplasm Translocation in Critical Limb Ischemia; Authors - Meixin Zhang
mouse	WB,IF	Sci Total Environ; Intestinal microbiota via NLRP3 inflammasome dependent neuronal pyroptosis mediates anxiety-like behaviour in mice exposed to 3.5 GHz radiofrequency radiation; Authors - Gui-Qiang Zhou
mouse	WB	Biochem Biophys Res Commun; Uric acid-lowering effect of harpagoside and its protective effect against hyperuricemia-induced renal injury in mice; Authors - Qiong Fu
mouse	WB	Food Sci Biotechnol; Geraniol alleviates liver injury induced by bisphenol A via modulating NLRP3/caspase-1 pathway and gut microbiota in mice model; Authors - Bangyuan Zhou