Ninein Monoclonal antibody

• Featured Product
• KD/KO Validated

Ninein Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Canine, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1B10A8

Reviews (2)

Cat no : 67132-1-Ig

Synonyms

GSK3B interacting protein, hNinein, KIAA1565, NIN, Ninein

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Validation Data Gallery

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  WB analysis using 67132-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67132-1-Ig (Ninein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67132-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67132-1-Ig (Ninein antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MDCK using 67132-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 67132-1-Ig (Ninein antibody) at dilution of 1:500 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, NCCIT cells, HT-29 cells
• Positive IHC detected in: mouse brain tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 2 publications below

Product Information

67132-1-Ig targets Ninein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Canine, mouse samples.

• Tested Reactivity: Human, Canine, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ninein fusion protein Ag14652
• Full Name: ninein (GSK3B interacting protein)
• Calculated Molecular Weight: 2090 aa, 243 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC034708
• Gene Symbol: Ninein
• Gene ID (NCBI): 51199
• RRID: AB_2882431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.

Publications

Species	Application	Title
human	WB,IF	Dev Cell; Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method; Authors - Sarah Carden ; KO Validated
human	IF	J Cell Sci; Roles for CEP170 in cilia function and dynein-2 assembly; Authors - Johannes F Weijman

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elisa (Verified Customer) (07-12-2021)

This antibody stains well ninein pool at the centrosome, in MetOH fixation conditions

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:300 IF 1:500WB
• Cell Tissue Type: HAP1, H9, RPE1 and HEK293T cells

FH

Sarah (Verified Customer) (07-06-2020)

Antibody used at 1:1000 to visualise ninein protein (from 20ug cell lysate) in Hap1 control and Ninein KO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hap1

Ninein Antibody Western Blot validation (1:1000 dilution) in Hap1 (Cat no:67132-1-Ig)