Ninein Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Ninein Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13007-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 13007-1-AP (same clone as 13007-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13007-1-AP (Ninein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13007-1-AP (same clone as 13007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13007-1-AP (same clone as 13007-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13007-1-AP (Ninein antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13007-1-AP (same clone as 13007-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13007-1-AP (same clone as 13007-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Ninein antibody (13007-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13007-1-PBS in a different storage buffer formulation.

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Product Information

13007-1-PBS targets Ninein in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ninein fusion protein Ag3688
• Full Name: ninein (GSK3B interacting protein)
• Calculated Molecular Weight: 2090 aa, 243 kDa
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC034708
• Gene Symbol: Ninein
• Gene ID (NCBI): 51199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ninein is a large coiled-coil protein that associates with the subdistal appendages of the mother centriole and the minus-ends of both centrioles. Ninein interacts with γ-tubulin and microtubule nucleation and anchoring at the centrosomes.