NeutraKine® IL-17F Monoclonal antibody

NeutraKine® IL-17F Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

1C7D10

Cat no : 69023-1-Ig

Synonyms

Cytokine ML 1, IL 17F, IL17F, IL-17F, interleukin 17F, ML 1, ML1

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Validation Data Gallery

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  Neutralization experiment of NeutraKine® IL-17F using 69023-1-Ig

  Recombinant human IL-17F (Cat.NO. HZ-1116) stimulates HT-1080 cells (human fibroblast cell line) produce IL-6 in a dose dependent manner (blue curve, refer to bottom X-Left Y axis). The activity of human IL-17F (500 ng/mL HZ-1116) is neutralized by mouse anti-human IL-17F monoclonal antibody 69023-1-Ig (red curve, refer to top X-right Y axis). The ND50 is typically 2-8 μg/mL.

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Neutralization

Product Information

69023-1-Ig targets NeutraKine® IL-17F in ELISA, Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine IL-17F protein HZ-1116
• Full Name: interleukin 17F
• Gene Symbol: IL-17F
• Gene ID (NCBI): 112744
• RRID: AB_2918843
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.

This antibody is used to neutralize the bioactivity of IL-17F.