NeutraKine® Cystatin C Monoclonal antibody

NeutraKine® Cystatin C Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

1H1B4

Cat no : 69017-1-Ig

Synonyms

ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, NeutraKine® Cystatin C, Post gamma globulin

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Validation Data Gallery

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  Neutralization experiment of NeutraKine® Cystatin C using 69017-1-Ig

  Recombinant human Cystatin C (Cat.NO. HZ-1211) inhibits Papain protease (5 μg/mL) activity (by colorimetric assay using L-BAPA as substrate) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human Cystatin C (0.4 μg/mL HZ-1211) is neutralized by mouse anti-human Cystatin C monoclonal antibody 69017-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is around 0.5mg/mL.

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Neutralization

Product Information

69017-1-Ig targets NeutraKine® Cystatin C in ELISA, Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine Cystatin C protein HZ-1211
• Full Name: cystatin C
• Gene Symbol: Cystatin C
• Gene ID (NCBI): 1471
• RRID: AB_2918837
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

This antibody can be used to neutralize the bioactivity of Cystatin C.