NeutraControl SCF Monoclonal antibody

NeutraControl SCF Monoclonal Antibody for ELISA, Non-Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Non-Neutralization

Conjugate

Unconjugated

CloneNo.

1E6G9

Cat no : 69530-1-Ig

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

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Validation Data Gallery

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  Non-Neutralization experiment of MO7e human megakaryoblastic leukemia cell line using 69530-1-Ig

  Recombinant human SCF (Cat.NO. HZ-1024) stimulates proliferation of the MO7e human megakaryoblastic leukemia cell line in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human SCF (30 ng/mL HZ-1024) is neutralized by mouse anti-human SCF monoclonal antibody 69030-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.2-1.0 μg/mL. The NeutraControl mouse anti-human SCF monoclonal antibody 69530-1-Ig could recognize human SCF, but could not neutralize human SCF in the range of 0.00244-40 μg/mL(green curve, refer to top X-right Y).

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  ELISA experiment of Recombinant protein using 69530-1-Ig

  Indirect ELISA was carried out by coating recombinant Human SCF (Cat.NO. HZ-1024) at 70 ng/well followed by blocking and adding serial diluted SCF antibody 69030-1-Ig and 69530-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Product Information

69530-1-Ig targets SCF in ELISA, Non-Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine SCF protein HZ-1024
• Full Name: KIT ligand
• Gene Symbol: SCF
• Gene ID (NCBI): 4254
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis.

This antibody is a neutralizing control antibody for SCF, the immunogen is the same as Neutrakine 69030-1-Ig but could not neutralize human SCF.