Neuropilin 2 Polyclonal antibody

Neuropilin 2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27193-1-AP

Synonyms

NRP2, Neuropilin-2

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Validation Data Gallery

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  WB analysis of U-87 MG using 27193-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U-87 MG using 27193-1-AP

  IP result of anti-Neuropilin 2 (IP:27193-1-AP, 4ug; Detection:27193-1-AP 1:500) with U-87 MG cells lysate 760 ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 27193-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 27193-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27193-1-AP (Neuropilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-87 MG cells
• Positive IP detected in: U-87 MG cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27193-1-AP targets Neuropilin 2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Neuropilin 2 fusion protein Ag25622
• Full Name: neuropilin 2
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC104770
• Gene Symbol: Neuropilin 2
• Gene ID (NCBI): 8828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuropilin 2 (NRP2) is a single-chain transmembrane glycoprotein that acts as a co-receptor of VEGF. NRP2 is mainly expressed in lymphatic and venous endothelial cells, and it is associated with the occurrence and development of several types of cancer. NRP2 is known to play a role in cardiovascular development, axon guidance, tumorigenesis, inflammation and cardiovascular disease.