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Neural Cell Markers Expanded Antibody Kit

Host/Isotype

Rabbit/Mouse

Reactivity

Target dependent

Applications

Target dependent

Conjugate

Unconjugated

Cat no : PK30027

Validation Data Gallery

Applications:
  • Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green).

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green).

  • Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MAP2 antibody (17490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue).

  • Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

    Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

  • Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66375-1-Ig (TUBB3-specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 26975-1-AP (NeuN antibody, red).

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66375-1-Ig (TUBB3-specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 26975-1-AP (NeuN antibody, red).

  • Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 66836-1-Ig (NeuN antibody, red), at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k antibody, green).

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using 66836-1-Ig (NeuN antibody, red), at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k antibody, green).

  • Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66836-1-Ig (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k Antibody, green).

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using 66836-1-Ig (NeuN antibody), at dilution of 1:100 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 14479-1-AP (Calbindin-D28k Antibody, green).

  • Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (81063-1-RR, Clone: 4C6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Myelin basic protein antibody (10458-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Myelin basic protein antibody (10458-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using S100 Beta antibody (66616-1-Ig, Clone: 1G10F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using S100 Beta antibody (66616-1-Ig, Clone: 1G10F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), NeuN antibody (66836-1-Ig, Clone: 3A4C1, red).

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using nestin antibody (29285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), NeuN antibody (66836-1-Ig, Clone: 3A4C1, red).

  • Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Nestin antibody (29285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Nestin antibody (29285-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Synaptophysin antibody (82900-1-RR, Clone: 2H14 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Synaptophysin antibody (82900-1-RR, Clone: 2H14 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSD95-Specific,DLG4 antibody (81106-1-RR, Clone: 1I22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSD95-Specific,DLG4 antibody (81106-1-RR, Clone: 1I22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17490-1-AP (MAP2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66836-1-Ig (NeuN antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens).

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81063-1-RR (GFAP antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10458-1-AP (Myelin basic protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10458-1-AP (Myelin basic protein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66616-1-Ig (S100 Beta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 82900-1-RR (Synaptophysin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Various lysates were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 17490-1-AP (MAP2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:49000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:49000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 81063-1-RR (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 10458-1-AP (Myelin basic protein antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 10458-1-AP (Myelin basic protein antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • rat brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66616-1-Ig (S100B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 29285-1-AP (nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 82900-1-RR (Synaptophysin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 82900-1-RR (Synaptophysin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Product Information

The Neural Cell Markers Expanded Antibody Kit provides a cost-effective tool for identifying and studying neuronal and glial cells. Perfect for researchers starting a new project, screening multiple prospective targets or those who simply require less volume.   

The Neural Cell Markers Expanded Antibody Kit contains antibodies against 12 key proteins for identifying neuronal and glial cells.

Antigen Catalog No. Host, clonality Tested Reactivity Applications Cell Type Marker Volume

MAP2

17490-1-AP             Rabbit polyclonal           H, M, Rt, Mk, G WB, IHC, IF/ICC, IF-P, FC, IP Neurons (dendrites) 20 uL

NeuN

66836-1-Ig             Mouse monoclonal  H, M, Rt, G        IHC, IF-P, FC Neurons (cell body) 20 uL

TUBB3

66375-1-Ig             Mouse monoclonal  H, M, Rt, Pg, Rb, Ch WB, IHC, IF/ICC, IF-P, FC Neurons (axon) 20 uL

GFAP
               

81063-1-RR             Rabbit Monoclonal H, M, Rt, Pg WB, IHC, IF-P Astrocytes 20 uL

Iba1

81728-1-RR             Rabbit Monoclonal             H, M, Rt           WB, IHC, IF-P, FC Microglia 20 uL            
CNPase 66729-1-Ig                 Mouse monoclonal  H, M, Rt, Pg WB, IF-P, FC Oligodendrocytes 20 uL                 
MBP 10458-1-AP               Rabbit polyclonal              H, M, Rt, Rb WB, IHC, IF-P Oligodendrocytes 20 uL                 
S100B 66616-1-Ig               Mouse monoclonal              H, M, Rt, Pg WB, IHC, IF-P Astrocytes 20 uL                 
Sox2 66411-1-Ig               Mouse monoclonal              H, M, Rt, Pg WB, IHC, IF/ICC, IF-P, FC Neural Stem/Progenitor Cells 20 uL                 
Nestin 29285-1-AP               Rabbit polyclonal              H, M, Rt WB, IF/ICC, IF-P, FC Neural Stem/Progenitor Cells 20 uL                 
Synaptophysin 82900-1-RR               Rabbit Monoclonal             H, M, Rt WB, IHC, IF/ICC, FC, IP Neurons (presynaptic) 20 uL                 
PSD-95 81106-1-RR               Rabbit Monoclonal             H, M, Rt WB, IHC, IF-P Neurons (postsynaptic)  20 uL                 

Also see our 'Neural Cell Markers Essentials Antibody Kit' on the following page  https://www.ptglab.com/products/Neural-Cell-Markers-Essentials-Antibody-Kit-PK30026.htm。

Storage

Store at -20°C. Stable for one year from the date of receipt. 

Background Information

The central nervous system (CNS) is a complex network consisting of a diverse subset of cells including neurons, astrocytes, oligodendrocytes, microglia, and neural stem cells. Identifying cell populations and protein localization can facilitate studies exploring the biological processes that occur during neurodevelopment or in neurological disorders, such as cell proliferation, migration, differentiation, morphology, and synapse formation.

Standard Protocols

Click   here  to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.

Cited in Article as

PK30027, Neural Cell Markers Expanded Antibody Kit, Proteintech, IL, USA