Nestin Monoclonal antibody, PBS Only

Nestin Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D11H2

Cat no : 66259-1-PBS

Synonyms

NES, Nestin

---

Validation Data Gallery

• 

  WB analysis of Neuro-2a using 66259-1-Ig (same clone as 66259-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66259-1-Ig (same clone as 66259-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 66259-1-Ig (same clone as 66259-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 66259-1-Ig (same clone as 66259-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66259-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66259-1-PBS targets Nestin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nestin
• Calculated Molecular Weight: 177 kDa
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: NM_006617
• Gene Symbol: NES
• Gene ID (NCBI): 10763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.