Nestin Monoclonal antibody

Nestin Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

27

CloneNo.

1D11H2

Cat no : 66259-1-Ig

Synonyms

NES, Nestin

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Validation Data Gallery

• 

  WB analysis of Neuro-2a using 66259-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66259-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 66259-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 66259-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66259-1-Ig (Nestin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Neuro-2a cells, HEK-293 cells, PC-12 cells, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 11 publications below
• FC: See 2 publications below

Product Information

66259-1-Ig targets Nestin in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nestin
• Calculated molecular weight: 177 kDa
• Observed molecular weight: 150-170 kDa
• GenBank accession number: NM_006617
• Gene symbol: NES
• Gene ID (NCBI): 10763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Publications

Species	Application	Title
human	IF	Cell Res; RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability.; Authors - Feng Xiong
rat	IF	Small; Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect.; Authors - Xiheng Lu
rat	IHC	J Cell Physiol; Specific inhibition of FAK signaling attenuates subchondral bone deterioration and articular cartilage degeneration during osteoarthritis pathogenesis.; Authors - Hangtian Wu
human		Front Oncol; Single-Cell Sequencing of Glioblastoma Reveals Central Nervous System Susceptibility to SARS-CoV-2.; Authors - Bingshan Wu
rat	IF	Sci Rep; Creation of X-linked Alport syndrome rat model with Col4a5 deficiency.; Authors - Masumi Namba
mouse	IF	Front Cell Neurosci; The Neural Stem Cell Properties of PKD2L1+ Cerebrospinal Fluid-Contacting Neurons in vitro.; Authors - Shuo Wang

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