Nephrin Polyclonal antibody

Nephrin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 22912-1-AP

Synonyms

NPHS1, Renal glomerulus-specific cell adhesion receptor, NPHS 1, NPHN, CNF

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Validation Data Gallery

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  WB analysis using 22912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22912-1-AP (Nephrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22912-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22912-1-AP (Nephrin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: rat kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

22912-1-AP targets Nephrin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nephrin fusion protein Ag18994
• Full Name: nephrosis 1, congenital, Finnish type (nephrin)
• Calculated Molecular Weight: 1241 aa, 135 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC156935
• Gene Symbol: Nephrin
• Gene ID (NCBI): 4868
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocytes are a major component of the glomerular blood filtration barrier. Podocyte protein Nephrin, also known as NPHS1, is the first to be identified as a central component of the slit diaphragm (SD), which is podocyte cell-cell junction. Mutations in Nephrin lead to congenital nephrosis, such as steroid-resistant proteinuria and congenital nephrotic syndrome of the Finnish type (CNF).

Publications

Species	Application	Title
mouse	WB	BMC Med Genomics; Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease; Authors - Binhui Pan
human	IHC	J Nephrol; Association of the podocyte phenotype with extracapillary hypercellularity in patients with diabetic kidney disease; Authors - Weixia Han
rat	IF	J Ethnopharmacol; Supplemented Gegen Qinlian Decoction Formula attenuates podocyte mitochondrial fission and renal fibrosis in diabetic kidney disease by inhibiting TNF-α-mediated necroptosis, compared with empagliflozin; Authors - Yu Wang
mouse	WB	Antioxidants (Basel); Identification and Validation of the miR/RAS/RUNX2 Autophagy Regulatory Network in AngII-Induced Hypertensive Nephropathy in MPC5 Cells Treated with Hydrogen Sulfide Donors; Authors - Qing Ye