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Nectin-4/PVRL4 Monoclonal antibody, PBS Only

Nectin-4/PVRL4 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4B6

Cat no : 67721-1-PBS

Synonyms

Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin-4, poliovirus receptor related 4, PRR4, PVRL4

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Nectin-4/PVRL4 Monoclonal antibody (67721-1-Ig)

    WB analysis of human placenta using 67721-1-Ig (same clone as 67721-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67721-1-Ig (Nectin-4/PVRL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67721-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Nectin-4/PVRL4 Monoclonal antibody (67721-1-Ig)

    WB analysis of A431 using 67721-1-Ig (same clone as 67721-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 67721-1-Ig (Nectin-4/PVRL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67721-1-PBS in a different storage buffer formulation.

Product Information

67721-1-PBS targets Nectin-4/PVRL4 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nectin-4/PVRL4 fusion protein Ag14414
Full Name poliovirus receptor-related 4
Calculated Molecular Weight 510 aa, 55 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC010423
Gene Symbol Nectin-4
Gene ID (NCBI) 81607
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).