Nectin-4/PVRL4 Polyclonal antibody

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  WB analysis using 31026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31026-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 31026-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31026-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 31026-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31026-1-AP (Nectin-4/PVRL4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 31026-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (31026-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Nectin-4/PVRL4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31026-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, nectin 4

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, MCF-7 cells, T-47D cells, MDA-MB-231 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31026-1-AP targets Nectin-4/PVRL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: poliovirus receptor-related 4
• Calculated Molecular Weight: 510 aa, 55 kDa
• Observed Molecular Weight: 55-80 kDa
• GenBank Accession Number: BC010423
• Gene Symbol: Nectin-4
• Gene ID (NCBI): 81607
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96NY8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a glycosylated form of 60-80 kDa and a deglycosylated form of 55-70 kDa. The 43 kDa band immunoprecipitated in the supernatant corresponds to the soluble Nectin-4 form. (PMID: 15784625).