Nectin-2/CD112 Recombinant antibody, PBS Only

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  WB analysis using 84460-5-RR (same clone as 84460-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84460-5-RR (Nectin-2/CD112 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84460-5-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 84460-5-RR (same clone as 84460-5-PBS)

  WB result of Nectin-2/CD112 antibody (84460-5-RR; 1:2000; room temperature for 1.5 hours) with wild-type and Nectin-2/CD112 knockout HAP1 cells. This data was developed using the same antibody clone with 84460-5-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 84460-5-RR (same clone as 84460-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Nectin-2/CD112 antibody (84460-5-RR, Clone: 241725D3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84460-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84460-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84460-5-RR against Human Nectin-2/CD112 were performed. The affinity constant is 0.314 nM.

• Featured Product
• KD/KO Validated

Nectin-2/CD112 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84460-5-PBS

Clone No.241725D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Nectin-2, Nectin 2, HVEB, Herpesvirus entry mediator B, Herpes virus entry mediator B

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Product Information

84460-5-PBS targets Nectin-2/CD112 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: NM_001042724.2
• Gene Symbol: Nectin-2/CD112
• Gene ID (NCBI): 5819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q92692-2
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.