Nectin-2/CD112 Polyclonal antibody

Nectin-2/CD112 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27171-1-AP

Synonyms

Nectin-2, Nectin cell adhesion molecule 2, Nectin 2, HVEB, Herpesvirus entry mediator B

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Validation Data Gallery

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  WB analysis using 27171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 27171-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 27171-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27171-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27171-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27171-1-AP (Nectin-2/PVRL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat kidney tissue, RAW 264.7 cells, mouse kidney tissue, Jurkat cells
• Positive IHC detected in: human tonsillitis tissue, human kidney tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

27171-1-AP targets Nectin-2/CD112 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nectin-2/CD112 fusion protein Ag26040
• Full Name: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC003091
• Gene Symbol: Nectin-2
• Gene ID (NCBI): 5819
• RRID: AB_2880784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nectin 2, also named as PVRL2, CD112, HVEB, PRR2 and PVRR2, is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111, CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V and C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses.

Publications

Species	Application	Title
mouse	IHC	Mucosal Immunol; Alpha-hemolysin of uropathogenic Escherichia coli induces GM-CSF-mediated acute kidney injury.; Authors - Changying Wang
rat	IF	J Transl Int Med; Lipid-related protein NECTIN2 is an important marker in the progression of carotid atherosclerosis: An intersection of clinical and basic studies.; Authors - Shen Li
human	IHC	iScience; Proteomic and single-cell landscape reveals novel pathogenic mechanisms of HBV-infected intrahepatic cholangiocarcinoma; Authors - Yifei Shen
human	IF	Sci Rep; Single-cell RNA sequencing unveils tumor heterogeneity and immune microenvironment between subungual and plantar melanoma; Authors - Panpan Wang
human	IF	Cancer Lett; Histone Lactylation Dynamics: Unlocking the Triad of Metabolism, Epigenetics, and Immune Regulation in Metastatic Cascade of Pancreatic Cancer; Authors - Xing Wang