ChromoTek Nano-CaptureLigand® mouse IgG1, Fc-specific VHH, biotinylated

The Nano-CaptureLigand® mouse IgG1 captures and immobilizes mouse IgG1 antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgG1 subclass with high affinity and in a site-directed manner.

Applications

BLI, SPR, ELISA

Conjugate

Biotin

Host

Alpaca

Reactivity

Mouse IgG1

Cat no : smsG1B-1

Synonyms

Nano-CaptureLigand® mouse IgG1, Fc domain, mouse IgG1, mouse immunoglobulin Gamma 1

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Validation Data Gallery

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  BLI binding kinetics of a mouse IgG1 anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal mouse IgG1 anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

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  BLI binding kinetics of a monoclonal rabbit IgG anti-DYKDDDDK-tag antibody to a DYKDDDDK-tagged protein. A monoclonal rabbit IgG anti-DYKDDDDK-tag antibody was immobilized using Nano-CaptureLigand human IgG/rabbit IgG, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of a DYKDDDDK-tagged protein.

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  BLI binding kinetics of a purified and a hybridoma supernatant mouse IgG1 anti-Halo antibody to Halo-tag. A purified, monoclonal mouse IgG1 anti-Halo antibody (blue, ChromoTek Halo antibody [28A8]) and a hybridoma supernatant, monoclonal mouse IgG1 anti-Halo antibody (green) were immobilized using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of Halo-tag protein.

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  ELISA capture of mouse IgG1 antibody using Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated. 50 nM Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Mouse IgG1 antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

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  Sandwich ELISA: Immobilization of the Nano-CaptureLigand on streptavidin coated microplates for ELISA. No biotinylation of the antibody is required. Coating of the streptavidin microplate with Nano-CaptureLigand (top left). Site-directed antibody immobilization via Nano-CaptureLigand (top right). Addition of sample including target (red, bottom left). Addition of detection and secondary antibody for read-out (bottom right).

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  Species reactivity of Secondary VHH.

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Product Information

The Nano-CaptureLigand® mouse IgG1 captures and immobilizes mouse IgG1 antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgG1 subclass with high affinity and in a site-directed manner.

• Description: The next level of antibody immobilization to BLI and SPR biosensors

  • Gentle, site-directed and Fc-specific immobilization of mouse IgG1 antibodies and Fc-fragments to streptavidin coated biosensors without biotinylation of the antibody

  • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)

  • High affinity for stable baseline without significant dissociation

  • >10x regeneration for multiple reuse

  • High specificity enables mouse IgG1 antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

• Applications: BLI, SPR, ELISA
• Host: Alpaca
• Reactivity: Mouse IgG1 Fc-fragment; No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3
• Target/Specificity: Fc-fragment of mouse IgG1
• Physical State: Liquid
• Conjugate: Biotin
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Nanobody
• Class: Recombinant
• Format: Alpaca single domain antibody, monovalent
• Cross-reactivity: No cross-reactivity to mouse IgG2a, Ig2b, IG2c, IgG3; human IgG1, IgG2, IgG3, IgG4, IgA, IgM, IgE; rabbit IgG; rat IgG1, IgG2a, IgG2b, IgG2c; sera from goat, guinea pig, human, macaque (cynomolgus monkey), rabbit, rat, sheep
• Clonality: Clonality
• Clones: CTK0103 (VHH0295)
• RRID: AB_2848186
• Affinity (KD): K_D=0.13 nM; Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
• Storage Buffer: 25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F
• Size: 10 μL; 100 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use