NWD1 Polyclonal antibody

NWD1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25025-1-AP

Synonyms

NWD1 Ens, NACHT domain- and WD repeat-containing protein 1

---

Validation Data Gallery

• 

  WB analysis of SH-SY5Y using 25025-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25025-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25025-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25025-1-AP (NWD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 25025-1-AP

  Immunofluorescent analysis of HepG2 cells using 25025-1-AP (NWD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, HepG2 cells, mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25025-1-AP targets NWD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NWD1 fusion protein Ag21754
• Full Name: NACHT and WD repeat domain containing 1
• Calculated Molecular Weight: 1564 aa, 175 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC117698
• Gene Symbol: NWD1
• Gene ID (NCBI): 284434
• RRID: AB_2879855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NWD1 also named as NWD1 Ens or NACHT and WD repeat domain containing 1 is a 1564 amino acid protein, which contains 1 NACHT domain and 13 WD repeat. The molecular weight of NWD1 was detected by western blot. The result shows that the molecular weight of NWD1 is 77 kDa., but NWD1 is a 174 kDa protein according to UniProt,. The function of NWD1 is still non-known, meanwhile the molecular weight of NWD1 is still remained to be confirmed.

Publications

Species	Application	Title
human	WB	EBioMedicine; Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.; Authors - Qin Yang