NVL Polyclonal antibody

NVL Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 16970-1-AP

Synonyms

nuclear VCP like, Nuclear VCP like protein, NVL, NVLp

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Validation Data Gallery

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  WB analysis using 16970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 16970-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16970-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16970-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16970-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16970-1-AP (NVL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16970-1-AP

  Immunofluorescent analysis of HepG2 cells using 16970-1-AP (NVL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse thymus tissue, mouse lung tissue, HeLa cells, mouse kidney tissue, rat thymus tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

16970-1-AP targets NVL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NVL fusion protein Ag9205
• Full Name: nuclear VCP-like
• Calculated Molecular Weight: 856aa,95 kDa; 659aa,73 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC012105
• Gene Symbol: NVL
• Gene ID (NCBI): 4931
• RRID: AB_2157811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NVL(nuclear VCP-like) belongs to the AAA ATPase family and may facilitate the dissociation and recycling of nucleolin, thereby promoting efficient ribosome biogenesis. It acts as an ATP-dependent zinc metallopeptidase. This protein has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
human	IF	J Cell Biol; Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases.; Authors - Yun Chawon C
human	WB,IF	Nat Commun; Brucella effectors NyxA and NyxB target SENP3 to modulate the subcellular localisation of nucleolar proteins; Authors - Arthur Louche

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (04-05-2020)

This antibody works okay. There are a few nonspecific bands, but I could tell which is the target band because I performed nuclear cytosolic fractionation. NVL is supposed to be in both nucleus and cytosol to shuttle ribosomes.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

NVL Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:16970-1-AP)