NUP85 Polyclonal antibody, PBS Only

NUP85 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15027-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 15027-1-AP (same clone as 15027-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15027-1-AP (NUP85 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 15027-1-AP (same clone as 15027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15027-1-AP (NUP85 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15027-1-AP (same clone as 15027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NUP85 antibody (15027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 15027-1-AP (same clone as 15027-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NUP85 antibody (15027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15027-1-PBS in a different storage buffer formulation.

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Product Information

15027-1-PBS targets NUP85 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP85 fusion protein Ag7158
• Full Name: nucleoporin 85kDa
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC000697
• Gene Symbol: NUP85
• Gene ID (NCBI): 79902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

This gene encodes a protein component of the Nup107-160 subunit of the nuclear pore complex. NPCs are key for nucleocytoplasmic transport, but also regulate the mitotic machinery, transcription and chromatin organization through transport-independent mechanisms. The NUP107-160 complex is acknowledged to contribute to the assembly and maintenance of the NPC structure. Members of this largest subcomplex of the NPC associate with kinetochores, mitotic spindles, centrosomes and mitotic checkpoint regulators for proper completion of the cell cycle . Downregulation of NUP107-160 subcomplex members resulted in defective cytokinesis, compromised microtubule structures, altered cytoskeletal dynamics, impaired chromosome segregation and differentiation. (PMID:34170319)