NUP62 Monoclonal antibody, PBS Only

NUP62 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G12B3

Cat no : 66573-1-PBS

Synonyms

62 kDa nucleoporin, IBSN, Nuclear pore glycoprotein p62, nucleoporin 62kDa, Nucleoporin Nup62, NUP62, p62, SNDI

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Validation Data Gallery

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  WB analysis using 66573-1-Ig (same clone as 66573-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66573-1-Ig (same clone as 66573-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66573-1-Ig (NUP62 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66573-1-Ig (same clone as 66573-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66573-1-Ig (same clone as 66573-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66573-1-Ig (NUP62 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66573-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66573-1-PBS targets NUP62 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NUP62 fusion protein Ag4912
• Full Name: nucleoporin 62kDa
• Calculated Molecular Weight: 522 aa, 53 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC014842
• Gene Symbol: NUP62
• Gene ID (NCBI): 23636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)