NUP62 Polyclonal antibody

• Featured Product
• KD/KO Validated

NUP62 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 13916-1-AP

Synonyms

62 kDa nucleoporin, IBSN, Nuclear pore glycoprotein p62, nucleoporin 62kDa, Nucleoporin Nup62, NUP62, p62, SNDI

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Validation Data Gallery

• 

  WB analysis of PC-3 using 13916-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13916-1-AP (NUP62 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13916-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13916-1-AP (NUP62 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 13916-1-AP

  IP result of anti-NUP62 (IP:13916-1-AP, 4ug; Detection:13916-1-AP 1:1000) with NIH/3T3 cells lysate 1200ug.

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Tested Applications

• Positive WB detected in: PC-3 cells, NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

13916-1-AP targets NUP62 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP62 fusion protein Ag4912
• Full Name: nucleoporin 62kDa
• Calculated Molecular Weight: 522 aa, 53 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC014842
• Gene Symbol: NUP62
• Gene ID (NCBI): 23636
• RRID: AB_2267660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)

Publications

Species	Application	Title
human	WB,IF	Nat Microbiol; Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.; Authors - Adarsh Dharan
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human	WB	PLoS Genet; Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.; Authors - Miao Chen
mouse	WB	iScience; Tat-P combined with GAPR1 releases Beclin1 to promote autophagy and improve Bronchopulmonary dysplasia model; Authors - Yahui Zhou
rat	WB	Pain; Transcriptional repression of the M channel subunit Kv7.2 in chronic nerve injury.; Authors - Rose Kirstin K
	WB	J Biol Chem; O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.; Authors - Li Liu