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NUP62 Polyclonal antibody

NUP62 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 13916-1-AP

Synonyms

SNDI, Nucleoporin Nup62, nucleoporin 62kDa, Nuclear pore glycoprotein p62, IBSN



Tested Applications

Positive WB detected inPC-3 cells, NIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13916-1-AP targets NUP62 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP62 fusion protein Ag4912
Full Name nucleoporin 62kDa
Calculated Molecular Weight 522 aa, 53 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC014842
Gene Symbol NUP62
Gene ID (NCBI) 23636
RRIDAB_2267660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)

Protocols

Product Specific Protocols
WB protocol for NUP62 antibody 13916-1-APDownload protocol
IHC protocol for NUP62 antibody 13916-1-APDownload protocol
IP protocol for NUP62 antibody 13916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Microbiol

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.

Authors - Adarsh Dharan
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
mouseWB

J Clin Invest

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Authors - Zhixiong Liu
humanWB

PLoS Genet

Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.

Authors - Miao Chen
mouseWB

iScience

Tat-P combined with GAPR1 releases Beclin1 to promote autophagy and improve Bronchopulmonary dysplasia model

Authors - Yahui Zhou
ratWB

Pain

Transcriptional repression of the M channel subunit Kv7.2 in chronic nerve injury.

Authors - Rose Kirstin K