NUP50 Polyclonal antibody

• Featured Product
• KD/KO Validated

NUP50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20798-1-AP

Synonyms

50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50

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Validation Data Gallery

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  WB analysis of Jurkat using 20798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20798-1-AP (NUP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20798-1-AP

  WB result of NUP50 antibody (20798-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells.

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  WB analysis of mouse brain using 20798-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20798-1-AP (NUP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20798-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

20798-1-AP targets NUP50 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP50 fusion protein Ag14710
• Full Name: nucleoporin 50kDa
• Calculated Molecular Weight: 468 aa, 50 kDa
• Observed Molecular Weight: 50 kDa, 65 kDa
• GenBank Accession Number: BC028125
• Gene Symbol: NUP50
• Gene ID (NCBI): 10762
• RRID: AB_10696184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L. This antibody recognizes both of two isoforms.

Publications

Species	Application	Title
human	WB	Oncogene; DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.; Authors - Bin Chen
rat	WB	J Cell Sci; Nucleoporin 50 mediates Kcna4 transcription to regulate cardiac electrical activity.; Authors - Xueting Gao ; KD Validated
human	WB	Nat Phys; Structural anisotropy results in mechano-directional transport of proteins across nuclear pores; Authors - Fani Panagaki ; KD Validated