NUP155 Monoclonal antibody

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  WB analysis of HEK293 cells using 66359-1-Ig

  WB result of NUP155 antibody (66359-1-Ig, 1: 6000) with si-Control and si-NUP155 transfected HEK293 cells.

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  WB analysis of HeLa using 66359-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66359-1-Ig (NUP155 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of ROS1728 using 66359-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66359-1-Ig (NUP155 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66359-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66359-1-Ig (NUP155 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66359-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66359-1-Ig (NUP155 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

NUP155 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66359-1-Ig

Clone No.1A7F2

Publications(4)

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

155 kDa nucleoporin, KIAA0791, N155, nucleoporin 155kDa, Nucleoporin Nup155, NUP155

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Tested Applications

• Positive WB detected in: HEK293 cells, HeLa cells, ROS1728 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

66359-1-Ig targets NUP155 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NUP155 fusion protein Ag3638
• Full Name: nucleoporin 155kDa
• Calculated Molecular Weight: 1391 aa, 155 kDa
• Observed Molecular Weight: 140-155 kDa
• GenBank Accession Number: BC039257
• Gene Symbol: NUP155
• Gene ID (NCBI): 9631
• RRID: AB_2881738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75694
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP155 is a nucleoporin, the major component of the nuclear pore complex (NPC) that is essential for nuclear envelope biogenesis. Mutation of NUP155 has been linked to cardiovascular disease.

Publications

Species	Application	Title
mouse	WB	Neuron; Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.; Authors - Zhixiong Liu
rat	WB	J Ethnopharmacol; Xinfuli granule alleviates metabolic remodeling through inhibition of endoplasmic reticulum stress and mitochondrial injury in heart failure; Authors - Yan-Ni Su
human	WB	BMC Cancer; Pan-cancer analysis of NUP155 and validation of its role in breast cancer cell proliferation, migration, and apoptosis; Authors - Zi-Qiong Wang ; KD Validated
human	WB	Front Pharmacol; NUP155 and NDC1 interaction in NSCLC: a promising target for tumor progression; Authors - Kai-Min Li