NUP133 Polyclonal antibody

NUP133 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12405-1-AP

Synonyms

133 kDa nucleoporin, hNUP133, nucleoporin 133kDa, Nucleoporin Nup133, NUP133

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Validation Data Gallery

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  WB analysis of HeLa using 12405-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12405-1-AP (NUP133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12405-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12405-1-AP (NUP133 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12405-1-AP

  IP result of anti-NUP133 (IP:12405-1-AP, 4ug; Detection:12405-1-AP 1:800) with HeLa cells lysate 880ug.

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  IHC staining of human colon cancer using 12405-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12405-1-AP (NUP133 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ChIP: See 1 publications below

Product Information

12405-1-AP targets NUP133 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUP133 fusion protein Ag3100
• Full Name: nucleoporin 133kDa
• Calculated Molecular Weight: 1156 aa, 129 kDa
• Observed Molecular Weight: 129 kDa
• GenBank Accession Number: BC020107
• Gene Symbol: NUP133
• Gene ID (NCBI): 55746
• RRID: AB_2154450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear pore complex protein Nup133 is a protein that in humans is encoded by the NUP133 gene. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.

Publications

Species	Application	Title
rat	WB	Int J Mol Sci; Fetal Zone Steroids and Estrogen Show Sex Specific Effects on Oligodendrocyte Precursor Cells in Response to Oxidative Damage.; Authors - Donna Elizabeth Sunny
human	ChIP	Mol Cell Pediatr; Nup133 and ERα mediate the differential effects of hyperoxia-induced damage in male and female OPCs.; Authors - Donna Elizabeth Sunny