NUMBL Polyclonal antibody

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  WB analysis of mouse brain using 10111-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 10111-1-AP

  WB result of NUMBL antibody (10111-1-AP, 1:3000) with si-Control and si-NUMBL transfected HeLa cells.

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  WB analysis of HeLa using 10111-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 10111-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10111-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10111-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10111-1-AP

  IP result of anti-NUMBL (IP:10111-1-AP, 4ug; Detection:10111-1-AP 1:1000) with HeLa cells lysate 2800 ug.

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  IHC staining of human brain using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10111-1-AP (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10111-1-AP (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10111-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse cerebellum using 10111-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NUMBL antibody (10111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 10111-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10111-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

NUMBL Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 10111-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

Numb-related protein, Numb-like protein, NUMBLIKE

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse spleen tissue, HeLa cells, mouse lung tissue, mouse liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue
• Positive IF/ICC detected in: PC-12 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below

Product Information

10111-1-AP targets NUMBL in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUMBL fusion protein Ag0156
• Full Name: numb homolog (Drosophila)-like
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC001794
• Gene Symbol: NUMBL
• Gene ID (NCBI): 9253
• RRID: AB_2154440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6R0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors.; Authors - Rasin Mladen-Roko MR
human	WB,IHC	Cancer Res; Regulation of lung cancer metastasis by Klf4-Numb-like signaling.; Authors - Vaira Valentina V ; KD Validated
human	WB,IHC	J Clin Endocrinol Metab; PMID: 16705073; Authors - Sinha-Hikim Indrani I
mouse	WB	Development; Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways.; Authors - Yusuke Okubo
mouse	IHC	Mol Endocrinol; Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation.; Authors - Moran Tyler B TB