NUMB Polyclonal antibody

NUMB Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29594-1-AP

Synonyms

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

---

Validation Data Gallery

• 

  WB analysis using 29594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29594-1-AP (NUMB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 29594-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using NUMB antibody (29594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29594-1-AP targets NUMB in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NUMB fusion protein Ag31553
• Full Name: numb homolog (Drosophila)
• Calculated Molecular Weight: 71KD
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: NM_001005743
• Gene Symbol: NUMB
• Gene ID (NCBI): 8650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUMB plays a role in the process of neurogenesis. It is required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. NUMB may also mediate local repair of brain ventricular wall damage. NUMB may play a role infurther understanding the NUMB function in development and cancer.