NUMB Polyclonal antibody

• Featured Product
• KD/KO Validated

NUMB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 18701-1-AP

Synonyms

h Numb, NUMB, numb homolog (Drosophila), Protein numb homolog, Protein S171, S171

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Validation Data Gallery

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  WB analysis using 18701-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18701-1-AP (NUMB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18701-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18701-1-AP (NUMB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18701-1-AP (NUMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18701-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18701-1-AP (NUMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

18701-1-AP targets NUMB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: numb homolog (Drosophila)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC068476
• Gene Symbol: NUMB
• Gene ID (NCBI): 8650
• RRID: AB_2154286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUMB, also named as S171, plays a role in the determination of cell fates during development. It is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The degradation of NUMB is induced in a proteasome-dependent manner by MDM2. Four transcript variants encoding different isoforms have been found for this gene. The antibody can bind to all these four isoforms P65, P66, P71 and P72, can't recognize the NUMBL.

Publications

Species	Application	Title
human	WB	Cancer Res; Regulation of lung cancer metastasis by Klf4-Numb-like signaling.; Authors - Vaira Valentina V ; KD Validated
human	WB	Cancer Lett; Therapeutic targeting m6A-guided miR-146a-5p signaling contributes to the melittin-induced selective suppression of bladder cancer.; Authors - Rucheng Yan
Human	IHC	Front Cell Dev Biol; IFT20 Mediates the Transport of Cell Migration Regulators From the Trans-Golgi Network to the Plasma Membrane in Breast Cancer Cells.; Authors - Huihui Yang
human	WB	Neurobiol Dis; The polarity protein Par3 regulates APP trafficking and processing through the endocytic adaptor protein Numb.; Authors - Miao Sun ; KD Validated
human	WB,IHC	J Cell Mol Med; Musashi2 predicts poor prognosis and invasion in hepatocellular carcinoma by driving epithelial-mesenchymal transition.; Authors - Lu He
human	WB	Oncol Lett; A gene mutation in RNA-binding protein 10 is associated with lung adenocarcinoma progression and poor prognosis.; Authors - Lin-Lin Yin