• Featured Product
  • KD/KO Validated

NUFIP1 Polyclonal antibody

NUFIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12515-1-AP

Synonyms

NUFIP, Nuclear FMRP-interacting protein 1, FMR1-interacting protein NUFIP1



Tested Applications

Positive WB detected inHeLa cells, A375 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12515-1-AP targets NUFIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUFIP1 fusion protein Ag3197
Full Name nuclear fragile X mental retardation protein interacting protein 1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC017745
Gene Symbol NUFIP1
Gene ID (NCBI) 26747
RRIDAB_2298759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of FMRP (Fragile X Mental Retardation Protein). FMRP is an RNA binding protein reported to be involved in translational control, notably at postsynaptic sites of protein synthesis as a part of a multiprotein/mRNA complex[PMID:12941608]. NUFIP1 is one of the several FMRP-interacting proteins. NUFIP can act as a pol II-specific basal transcriptional activator in vitro and when ectopically overexpressed in vivo. NUFIP can directly activate promoters by enhancing the ATP-dependent release of hyperphosphorylated form of pol II from open transcription complexes[PMID:15107825].

Protocols

Product Specific Protocols
WB protocol for NUFIP1 antibody 12515-1-APDownload protocol
IHC protocol for NUFIP1 antibody 12515-1-APDownload protocol
IF protocol for NUFIP1 antibody 12515-1-APDownload protocol
IP protocol for NUFIP1 antibody 12515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Authors - Heeseon An
  • KO Validated
humanWB

Science

NUFIP1 is a ribosome receptor for starvation-induced ribophagy.

Authors - Gregory A Wyant
  • KO Validated
mouseWB

Sci Adv

The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.

Authors - Pedro Fuentes
  • KD Validated
humanWB,IF

Autophagy

The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress.

Authors - Myoung Sup Shim
humanWB

J Cell Biol

Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

Authors - Jonathan Bizarro
humanWB

Nucleic Acids Res

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Authors - Anne-Sophie Gribling-Burrer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

H (Verified Customer) (04-06-2020)

This antibody is okay, but there are multiple off-target bands especially in the nucleus. I have performed immunofluorescence analysis using KO cells, and I found significant signal in the nucleus even in the KO cells, requiring caution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T, HEK293 and HCT116
NUFIP1 Antibody Western Blot validation (1:1000 dilution) in HEK293T, HEK293 and HCT116 (Cat no:12515-1-AP)